直播推薦
企業(yè)動態(tài)
- 2025 廣州國際分析測試及實(shí)驗(yàn)室設(shè)備展覽會暨技術(shù)研討會即將圓滿結(jié)束
- 華測儀器推出全新脈沖發(fā)生器系列
- 智易時(shí)代參與編制的水質(zhì)監(jiān)測團(tuán)標(biāo)證書正式下發(fā)!
- 上海佳航邀請您參加2025廣州國際分析測試及實(shí)驗(yàn)室設(shè)備展覽會暨技術(shù)研討會
- 科研新突破!司老師團(tuán)隊(duì)與華測儀器合作論文發(fā)表
- 光耀廿七載 智啟新未來 | 熱烈慶祝東潤儀表成立27周年
- 中研股份與華測儀器正式簽訂Huace-300表面體積電阻率測試儀合作協(xié)議
- 云南某商貿(mào)公司采購聚同人工氣候箱一臺
推薦展會
SW579(人甲狀腺癌細(xì)胞)
Cell Biology
ATCC® Number: HTB-107™ Price:
Designations: SW579 [SW 579; SW-579] Depositors: A Leibovitz
Biosafety Level: 1 Shipped: frozen
Medium & Serum: See Propagation Growth Properties: adherent
Organism: Homo sapiens (human) Morphology: epithelial
Source: Organ: thyroid
Disease: squamous cell carcinoma
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Related Cell Culture Products
Isolation: Isolation date: 1973
Tumorigenic: Yes, forms tumors in nude mice
(The cells produce grade III malignant spindle and giant cell tumors.)
Antigen Expression: blood type O; Rh+
DNA Profile
(STR): Amelogenin: X
CSF1PO: 13
D13S317: 13
D16S539: 11
D5S818: 11
D7S820: 8,9
THO1: 8,9.3
TPOX: 8,10
vWA: 14,18
Isoenzymes: AK-1, 1; ES-D, 1; G6PD, B; GLO-I, 2; Me-2, 1-2; PGM1, 1-2; PGM3, 1
Age: 59 years
Gender: male
Ethnicity: Caucasian
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0C
Atmosphere: air, 100%
Subculturing: Protocol:
Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37C.
Subc*tion ratio: A subc*tion ratio of 1:5 to 1:10 is recommended
Medium renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium): ATCC 30-2008
recommended serum: ATCC 30-2020
References: 22536: Fogh J , et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871
22539: Fogh J , et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080
免責(zé)聲明
- 凡本網(wǎng)注明“來源:儀器網(wǎng)”的所有作品,均為浙江興旺寶明通網(wǎng)絡(luò)有限公司-儀器網(wǎng)合法擁有版權(quán)或有權(quán)使用的作品,未經(jīng)本網(wǎng)授權(quán)不得轉(zhuǎn)載、摘編或利用其它方式使用上述作品。已經(jīng)本網(wǎng)授權(quán)使用作品的,應(yīng)在授權(quán)范圍內(nèi)使用,并注明“來源:儀器網(wǎng)”。違反上述聲明者,本網(wǎng)將追究其相關(guān)法律責(zé)任。
- 本網(wǎng)轉(zhuǎn)載并注明自其他來源(非儀器網(wǎng))的作品,目的在于傳遞更多信息,并不代表本網(wǎng)贊同其觀點(diǎn)和對其真實(shí)性負(fù)責(zé),不承擔(dān)此類作品侵權(quán)行為的直接責(zé)任及連帶責(zé)任。其他媒體、網(wǎng)站或個(gè)人從本網(wǎng)轉(zhuǎn)載時(shí),必須保留本網(wǎng)注明的作品第一來源,并自負(fù)版權(quán)等法律責(zé)任。
- 如涉及作品內(nèi)容、版權(quán)等問題,請?jiān)谧髌钒l(fā)表之日起一周內(nèi)與本網(wǎng)聯(lián)系,否則視為放棄相關(guān)權(quán)利。
2025第13屆武漢國際科學(xué)儀器與實(shí)驗(yàn)室裝備展覽會
展會城市:武漢市展會時(shí)間:2025-03-20